Resumo. Interspecific interactions among ant species influence community organization, especially when highly aggressive species are involved. Solenopsis invicta is well known for its aggressiveness, while Atta laevigata plays a crucial role as a leaf-cutting ant in the Cerrado. This study documents and analyzes an event of collective aggression by S. invicta against a recently fertilized founding queen of A. laevigata in a legal reserve area in southern Tocantins, Brazil. Following the nuptial flight of A. laevigata, a field observation directly recorded an incident in which S. invicta workers attacked the individual. The attack involved continuous aggressive actions, repeated biting, and worker recruitment, resulting in evident debilitation. This record shows that S. invicta can act as a mortality agent for Atta foundresses in preserved natural environments, thereby enhancing the understanding of ecological interactions among ants in the Cerrado.