Resumo. One possibility for the value addition of agro-industrial waste is its use for the growth of edible mushrooms of the genus Pleurotus. The objective of this work was to evaluate the productive parameters and nutritional value of Pleurotus spp. cultivated in agro-industrial wastes available in the northeastern region of the State of Santa Catarina. Pleurotus ostreatus, Pleurotus sajor-caju and Pleurotus djamor were cultivated using banana pseudostem and onion waste as substrates. Yield (Y%), biological efficiency (BE%) and productivity (Pr g/day) were evaluated. The fruiting bodies obtained from the substrate with the best Y% and BE% were analyzed for moisture, ash, carbohydrates, fibers, lipids and proteins. P. ostreatus has shown the highest Y% (126%) when cultivated in onion waste. P. sajor-caju also obtained the highest BE% (10,8%) and Pr (0.43 g/day) in onion waste. P. ostreatus cultivated in onion waste has shown higher amounts of carbohydrates (63.7%) and proteins (26.2%) than those observed in the literature, while the level of fat, fiber and energy value, when in banana pseudostem, were higher (7.4%, 14.7% and 402.4 kcal/100 g, respectively). The results showed that onion and banana pseudostem wastes are efficient substrates for the growth of P. ostreatus and P. sajor-caju.