Resumo. Abstract Aim The following list aims to provide the most recent taxonomic surveying of fish species inhabiting the Ivaí and the Pirapó/Parapanema river basins from the Maringá municipality, using material from the Coleção Ictiológica do Nupélia (NUP) and the available literature. Methods We compiled all the available records of fish species housed in the NUP from the SpeciesLink database, and compared them with those in a previously published study. Results The total number of species identified was 81, distributed across 24 families and six orders. The richest order is Characiformes, comprising 34 species. The data analysed reveal that 11 species are recorded as non-native, highlighting the alarming case of introduced species, especially by aquarium keeping. Regarding the threat categories analysed by the IUCN, only one species, Bryconamericus coeruleus, is considered Near Threatened. Further, a noteworthy feature is the presence of seven putative new species, which underlines the necessity for further studies to be conducted to facilitate a more complete understanding and description of the ichthyofauna of the region. The results of this study show that the fish species composition in the streams of the Maringá municipality is diverse and taxonomically complex. Conclusions Further investigation is needed to address the aforementioned issues regarding conservation and possible species description. Furthermore, more surveys are needed in the Ivaí River basin to expand the species registry, especially in urban streams. We hope that the list of freshwater fish species from Maringá municipality will be a useful source of information for people who make decisions and for people who want to create plans to manage and protect the fish in the region.