Resumo. Hematophagous ceratopogonid biting midges are small dipterans of medical and veterinary importance, capable of causing severe discomfort to humans, domestic animals, ruminants, and wildlife and vectors of several etiological agents. Despite their importance as pollinators and vectors of pathogens affecting vertebrates, Ceratopogonidae remains a neglected family, particularly in the Amazon Basin. This study reports biting midges collected the Lower Amazon and Southwestern Pará regions, Brazil. Collections were carried out near peridomestic areas using CDC light traps and entomological sweep nets. A total of 6,033 biting midges were collected. Of these, 15.6% (941 specimens) belonged to the genus Leptoconops Skuse, 1889, represented by one species, while 84.4% (5,092 specimens) belonged to the genus Culicoides Latreille, 1809, represented by 12 species. New records were found for the State of Pará: Culicoides rhombus Santarém, Felippe-Bauer & Castellón, 2014 and Culicoides kuripako Felippe-Bauer, 2010. Species of medical and veterinary importance were found in the study, Culicoides insignis Lutz, 1913 and Culicoides paraensis (Goeldi, 1905). This species list is valuable for identifying the biting midge fauna of the Lower Amazon and Southwestern Pará, as it provides data on species identification and distribution. It is also important for epidemiological studies and vector control.